10 mM Tris, 0.1% Sodium Azide, pH 8.0 ± 0.1
This is our CEA & PSA Dilution Buffer, designed for certain preparations of our CEA (Cat. No. C0224) PSA (Cat. Nos. P0723, P0724, P0725, P0715), and Non-Complexing PSA (Cat. No. P5125), but it is a suitable Tris buffer for use when no additional buffer salts are required. Buy this buffer
Ingredients Tris Base: 1.210 g/L Sodium Azide: 1.0 g/L 5M HCl: As needed 5M NaOH: As needed Distilled Water (dH2O)
Directions 1. Dissolve Tris Base and Sodium Azide in approx. 90% of final volume with cold dH2O. 2. Add cold dH2O to obtain final volume. 3. Adjust to pH 8.0 ± 0.1 with 5M HCl or 5M NaOH as needed. Filter as needed.
10 mM Tris, 150 mM Sodium Chloride, 0.1% Sodium Azide, pH 8.0 ± 0.1
This is our Ferritin Dilution Buffer, designed for our Liver Ferritin (Cat. No. F0324) and Spleen Ferritin (Cat. No. F0424), but it is a suitable Tris buffer for use when additional buffer salts are needed. Buy this buffer
Ingredients Tris HCl: 1.296 g/L Tris Base: 0.215 g/L Sodium Chloride: 8.766 g/L Sodium Azide: 1.0 g/L 5M HCl: As needed 5M NaOH: As needed Distilled Water (dH2O)
Directions 1. Dissolve Tris HCl, Tris Base, Sodium Chloride, and Sodium Azide in approx. 90% of final volume with cold dH2O. 2. Adjust to pH 8.0 ± 0.1 with 5M HCl or 5M NaOH as needed. 3. Add cold dH2O to obtain final volume. 4. Check pH and adjust to pH 8.0 ± 0.1 with 5M HCl or 5M NaOH as needed.
20 mM Tris, 1 mM EDTA, 50% Glycerol, pH 8.5 ± 0.1
This is our Myoglobin Dilution Buffer, designed for our Cardiac Myoglobin (Cat. Nos. M0724, M0725) and Skeletal Myoglobin (Cat. No. M0224), but it is a suitable Tris buffer for use when the additional stability provided by EDTA and glycerol are needed. Buy this buffer
Ingredients Tris HCl: 1.950 g/L Tris Base: 0.920 g/L EDTA, Tetrasodium: 0.416 g/L Glycerol: 500 ml/L 5M HCl: As needed 5M NaOH: As needed Distilled Water (dH2O)
Directions 1. Dissolve Tris HCl, Tris Base, and EDTA in approx. 45% of final volume with cold dH2O. 2. Adjust to pH 8.5 ± 0.1 with 5M HCl or 5M NaOH as needed. 3. Add cold dH2O to obtain approx. 50% of final volume. 4. Add cold Glycerol to obtain final volume. Mix thoroughly and chill. 5. Check pH and adjust to pH 8.5 ± 0.1 with 5M HCl or 5M NaOH as needed.
20 mM Tris, 150 mM Sodium Chloride, pH 7.4 ± 0.1
This is our ACT Dilution Buffer, designed for our a1 Antichymotrypsin (Cat. No. A1824). This solution is similar to our Ferritin Dilution Buffer, but with a stronger Tris concentration and a slightly lower pH. Buy this buffer
Ingredients Tris Base: 2.420 g/L Sodium Chloride: 8.766 g/L 5M HCl: As needed 5M NaOH: As needed Distilled Water (dH2O)
Directions 1. Dissolve Tris Base and Sodium Chloride in approx. 90% of final volume with cold dH2O. 2. Adjust to pH 7.4 ± 0.1 with 5M HCl as needed. 3. Add cold dH2O to obtain final volume and chill. 4. Check pH and adjust to pH 7.4 ± 0.1 with 5M HCl or 5M NaOH as needed. 5. Filter using 0.45 µm filter unit.
20 mM Tris, 280 mM Sodium Chloride, 5 mM Calcium Chloride, 0.1% Sodium Azide, pH 8.0 ± 0.1
This is our CRP Dilution Buffer, designed for our highly purified C-Reactive Protein (Cat. No. C0129), but it is suitable for use when a stronger concentration of Tris and buffer salts, plus calcium chloride, are needed. Buy this buffer
Ingredients Tris Base: 2.423 g/L Sodium Chloride: 16.363 g/L Calcium Chloride: 0.735 g/L Sodium Azide: 1.0 g/L 5M HCl: Approx. 3 ml/L 5M NaOH: As needed Distilled Water (dH2O)
Directions 1. Dissolve Tris Base and Sodium Chloride in approx. 90% of final volume with cold dH2O. 2. Adjust to pH 8.0 ± 0.1 with 5M HCl as needed. 3. Add cold dH2O to obtain final volume and chill. 4. Check pH ,adjust to pH 8.0 ± 0.1 with 5M HCl or 5M NaOH as needed, then add Sodium Azide. 5. Add Calcium Chloride and allow to dissolve completely. (IMPORTANT: The pH CANNOT be adjusted after adding Calcium Chloride.) 5. Filter using 0.2 µm filter unit.
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