Recombinant beta-hCG
RECOMBINANT ß-HCG DATA
(View data as PDF - available soon)
Recombinant Human Chorionic Gonadotropin, Beta-Subunit (ß-hCG) from Scripps Laboratories is available for research use and diagnostic assay development. A global shortage in the supply of starting material for ß-hCG severely impacted the availability of this critical reagent to the diagnostic industry. Scripps Laboratories offers highly purified recombinant ß-hCG as a reliable and economical alternative to native ß-hCG. The data presented here demonstrate its suitability for assay development and large-scale manufacturing of diagnostic assays.
SDS-PAGE
Figure 1 presents an SDS-PAGE of Recombinant ß-hCG. Gel staining indicates the protein is highly purified and runs at the expected molecular weight for glycosylated ß-hCG. In addition, Recombinant ß-hCG contains no visible contaminating proteins in the reduced/heated (lanes 1,2) and non-reduced/not heated (lanes 6,7) samples.
WESTERN BLOT
Western blot analysis demonstrates Recombinant ß-hCG is antibody-reactive under a variety of conditions. The data in Figure 2 show it retains its antibody-binding capabilities under several combinations of reduced/non-reduced and heated/not heated treatments. The gel was stained with a Scripps Laboratories ß-hCG monoclonal antibody, clone BC001, Mat. No. MC097-90000.
SDS-PAGE, GLYCOPROTEIN STAIN
Native hCG is a glycoprotein produced and glycated in the trophoblastic cells of the placenta during pregnancy. Both the α- and ß-subunits of hCG are known to be highly glycosylated. Recombinant ß-hCG from Scripps Laboratories is produced in a mammalian cell line and the image at right confirms it is glycosylated. Figure 3 is an SDS-PAGE of Recombinant ß-hCG, stained for glycoprotein. The gel shows significant glycosylation, as expected.
HPLC
Analyzed by HPLC, the profile of Recombinant ß-hCG in Figure 4 displays a single main peak with a retention time of 19.765 min. The profile confirms what is corroborated by SDS-PAGE: Recombinant ß-hCG is purified to a high extent and contains no significant contaminants.
The data presented here demonstrate Recombinant ß-hCG is highly purified, glycosylated, and antibody reactive. It is an economical and sustainable alternative to native ß-hCG and is suitable for research use or diagnostic assay development. In addition, it has the added benefit of being produced in a sustainable, recombinant system.
Recombinant ß-hCG is in stock and available now: