This solution is provided in a ready-to-use format and must not be diluted. The substrate is light sensitive and must be protected from light during incubation with the alkaline phosphatase-labeled probe.
BCIP/NBT Substrate is a ready-to-use single component substrate intended for the detection of alkaline phosphatase-labeled probes on membranes (e.g. PCR, western blot, dot blot, etc.). The substrate is a stabilized formulation of BCIP (5-Bromo-4-Chloro-3-Indoly-Phosphate) and NBT (NitroBlue Tetrazolium chloride). This solution may be used in blot applications as a substitute for 2-component substrates. After incubating with the alkaline phosphatase-labeled probe and washing with Tris buffer, add substrate to completely cover the membrane. Incubate membrane until a sufficient color intensity appears (typically 5-20 minutes) and stop the reaction by thoroughly washing with distilled or deionized water.
Precaution: Do Not Dilute! This solution is provided “ready-to-use” and may not perform correctly if diluted. The substrate is light sensitive and must be protected from light during incubation with the alkaline phosphatase-labeled probe.
Scripps Laboratories is a leading producer and supplier of BCIP/NBT Substrate. Products are prepared on-site and shipped to you directly. Custom pack sizes are available. For Bulk Discounts or additional product information, please use the button below to send us a note.