Recombinant hLH
Recombinant hLH, CHO & HEK 293
Recombinant Human Luteinizing Hormone (hLH) from CHO and HEK 293 cells are offered as high-quality replacements for native hLH. Starting materials for native proteins continue to be in short supply, so Scripps Laboratories is producing renewable, recombinant alternatives to address this global concern. Use the links below to view data which demonstrate that Recombinant hLH-CHO and hLH-HEK 293 possess the characteristics required to make them suitable replacements for the native proteins.
Recombinant hLH, CHO
View data as a PDF: Recombinant hLH data
View product: Recombinant hLH-CHO, ≥95%
Recombinant hLH, HEK 293
View data as a PDF: Recombinant hLH data
View product: Recombinant hLH-HEK 293, ≥95%
RECOMBINANT hLH DATA
Recombinant Human Luteinizing Hormone (hLH) is now available from Scripps Laboratories for research use and diagnostic assay development. The ongoing global shortage in the supply of human pituitary glands has severely impacted the availability of native hLH to the diagnostic industry. In response to this, Scripps developed Recombinant hLH from CHO and HEK 293 cells. Both are reliable and economical alternatives to native hLH. The data presented here demonstrate their suitability for research, assay development and large-scale assay manufacturing purposes.
SDS-PAGE
Figure 1 presents SDS-PAGE images of purified Recombinant hLH from CHO cells (left image) and Recombinant hLH from HEK 293 cells (right image). Both forms of Recombinant hLH run at the expected molecular weights under reduced/heated, non-reduced/heated, and non-reduced/not heated conditions. (See Figure 1 caption for details.) In addition, no discernable contaminants are visible in either gel.
SDS-PAGE, GLYCOPROTEIN STAIN
Native hLH is a glycoprotein produced and secreted by the anterior lobe of the pituitary gland. Scripps Laboratories offers two forms of Recombinant hLH from mammalian cell lines, CHO and HEK 293, and both forms are also glycoproteins. Figure 2 presents SDS-PAGE gels for Recombinant hLH-CHO and Recombinant hLH-HEK 293, stained for carbohydrate moieties. Separation of the alpha- and beta-subunits of both forms of Recombinant hLH can be seen and all subunits are confirmed to be glycosylated.
HPLC
The HPLC profiles of Recombinant hLH-CHO (top) and Recombinant hLH-HEK 293 (bottom) are shown in Figure 3. Both profiles display prominent single peaks with similar retention times of 20.389 min. (Recombinant hLH-CHO) and 20.705 min. (Recombinant hLH-HEK 293). The absence of any minor peaks confirms what is corroborated by SDS-PAGE, that both recombinant proteins are highly purified with no discernable contaminants.
The data presented here for Recombinant hLH-CHO and Recombinant hLH-HEK 293 indicate they are excellent alternatives to native hLH. Produced in mammalian cell culture systems, both recombinants are highly-purified, glycosylated proteins. Bulk quantities are available for large-scale manufacturing with excellent lot-to-lot consistency. Use the links below to learn more.
Recombinant hLH is in stock and available now: