BCIP/NBT Substrate
$50.00
- SKU:
- 90537-0100
- Catalog Number:
- B0522
- Part Number:
- 90537
- Physical Form:
- Liquid
- Pricing:
- Note that non-USA, export shipments will incur applicable charges, such as freight, customs, duties, VAT, or other fees associated with product importation. Pricing is in US Dollars.
This alkaline phosphatase substrate is provided in a ready-to-use format and does not need to be diluted. It is a single component substrate and intended for use in PCR, western blot, dot blot, and other membrane-based assay systems. The substrate is light sensitive and must be protected from light during incubation with the alkaline phosphatase-labeled probe.
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| Catalog Number | B0522 | |
| Part Number | 90537 | |
| Characteristics Specification |
BCIP / NBT Substrate is a ready-to-use single component substrate intended for the detection of Alkaline Phosphatase labeled probes on membranes (e.g. western blots, dot blots). The substrate is a stabilized formulation of BCIP (5-bromo-4-Chloro-3-Indoly-Phosphate-p-toluidine ) and NBT (NitroBlue Tetrazolium chloride). This solution may be used in blot applications as a substitute for 2-component substrates. After incubating with the Alkaline Phosphatase labeled probe and washing with Tris buffer, add substrate to completely cover the membrane. Incubate membrane until a sufficient color intensity appears (typically 5 - 20 minutes) and stop the reaction by thoroughly washing with distilled or deionized water. | |
| Storage / Shipping | Product is shipped ambient. On arrival store refrigerated at 2°C to 8°C. Do not freeze! | |
| Precaution / Hazard Statement |
Do Not Dilute! Solution is provided "ready-to-use" and may not perform correctly if it is diluted. The substrate is light sensitive and must be protected from light during incubation with the Alkaline Phosphatase-labeled probe. |
