This preparation of Recombinant Human Thyroid Stimulating Hormone (Rec. hTSH) is a glycosylated, whole molecule form of hTSH and it is reactive in a variety of antibody-based assay systems. It is produced in a mammalian cell line and purified by column chromatography. It is provided as a powder, lyophilized from ammonium bicarbonate. Protein content is determined using the Lowry Protein Assay (BSA standard).
Reconstitution: Conditions of reconstitution may vary based on your system, but we recommend using the following buffer: 10 mM Sodium Phosphate, 150 mM Sodium Chloride, 1 mg/ml BSA, 0.1% Sodium Azide (optional), pH 7.4. A detailed description of our recommended reconstitution protocol is offered here.
NOTE: To assure product integrity, we recommend using a recently calibrated pH meter to assure accurate pH determination.
As a PRIMARY MANUFACTURER, Scripps is able to offer research-scale quantities of high quality antigens and antibodies, as presented on this website, yet also large, manufacturing-scale quantities. Materials are produced, packaged, and shipped from our facility in San Diego, California.