This preparation of bovine TSH is isolated from bovine pituitary glands and is provided as a powder, lyophilized from ammonium bicarbonate. Protein concentration prior to lyophilization is determined using the Lowry Protein Assay with BSA standards. Supplemental reporting includes Bovine TSH content by ELISA.
Bovine TSH from Scripps is not assigned a Unit Activity. Previously available preparations of bovine TSH reported Unit Activity as determined by the Lamberg assay (1953). The assay, which involved measuring the uptake of radioactive 32P by the thyroid gland of young chickens, is no longer in use and, therefore, a Unit Activity is not reported for this product. Scripps has, however, performed some comparative evaluations and found that 100 μg of the current bovine TSH product is equivalent to approximately 3 Units of the old material.
As a PRIMARY MANUFACTURER, Scripps is able to offer research-scale quantities of high quality antigens and antibodies, as presented on this website, yet also large, manufacturing-scale quantities. Materials are produced, packaged, and shipped from our facility in San Diego, California.