Scripps Laboratories has several PSA antibodies available, including four monoclonals and one goat polyclonal. Monoclonals are available that detect PSA and complexed PSA, as well as a monoclonal that is specific for free, unbound PSA. Matched pairs of antibodies are described immediately below. In addition, some of our clones have an inhibitory effect on the chymotrypsin-like enzymatic activity of PSA. These qualities and the binding characteristics of each antibody are presented in the Reactivity Descriptions table below.
PSA ANTIBODY PAIRS
• MP077-90197, Clone AP002S pairs with MP077-90205, Clone BP005S to detect Total PSA
• MP077-90198, Clone AP003S pairs with MP077-90205, Clone BP005S to detect Free PSA
• MP077-90201, Clone BP001 pairs with Goat anti-PSA, GP079-90129 to detect Total PSA
Reagents Used For Free PSA and Total PSA ELISA Evaluations:
• Total PSA Monoclonal: MC097-90197, Clone AP002S
• Total PSA Monoclonal: MC097-90205, Clone BP005S
• Free PSA Monoclonal: MC097-90198, Clone AP003S
• PSA Antigen: P0725-90017
• PSA-ACT Complex: P0625-90220
PSA ANTIBODY REACTIVITY DESCRIPTIONS
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Material No.
|
Antibody Type
|
Characteristics
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|---|
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MP077-90197
Clone AP002S
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Mouse Monoclonal
IgG1
|
- Recognizes PSA, PSA/ACT complex, and PSA/A2M complex by direct ELISA and western blot.
- Sandwich ELISA for PSA:PSA/ACT gives an equimolar response when paired with clone BP005S.
- This clone does not significantly inhibit the chymotrypsin-like activity of PSA.
|
|
MP077-90198
Clone AP003S
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Mouse Monoclonal
IgG1
|
- Recognizes only free PSA by direct ELISA and western blot.
- Suitable for use in a sandwich ELISA for free PSA when paired with clone BP005S.
- Greater than 98% inhibition of PSA chymotrypsin-like activity at a 1:2 molar ratio of PSA:MAb.
|
|
MP077-90201
Clone BP001
|
Mouse Monoclonal
IgG1,k
|
- Recognizes PSA and PSA/ACT complex by direct ELISA and western blot.
|
|
MP077-90205
Clone BP005S
|
Mouse Monoclonal
IgG1,k
|
- Recognizes PSA, PSA/ACT complex, and PSA/A2M complex by direct ELISA and western blot.
- Sandwich ELISA for PSA:PSA/ACT gives an equimolar response when paired with clone AP002S.
- Greater than 95% inhibition of PSA chymotrypsin-like activity at a 1:2 molar ratio of PSA:MAb.
|
|
GP079-90129
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Goat Polyclonal
_____________ |
- Affinity purified on a column containing immobilized human PSA.
- Purity is ≥95% by SDS-PAGE analysis and the antibody shows a single arc by immunoelectrophoresis (IEP) against purified human PSA.
- There is no reaction against 2X concentrated human plasma.
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