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Creatine Kinase MB, Recombinant

$150.00
SKU:
90673-0100
Catalog Number:
C1232
Part Number:
90673
Physical Form:
Clear liquid
Purity:
Partially pure. Purity reported by SDS-PAGE.
Creatine Kinase Purity:
≥99% CK-MB by agarose electrophoresis when stained for creatine kinase activity.

This preparation of Recombinant Creatine Kinase MB (CK-MB) is the two-subunit molecule, composed of the creatine kinase M and B proteins, and it is partially purified. It is produced in E. coli and is provided as a liquid in 5 mM Sodium Succinate, 10 mM Sodium Chloride, 1 mM EDTA, 5 mM β-Mercaptoethanol, 50% Glycerol, pH 7.0. Purity is ≥99% CK-MB by agarose electrophoresis when stained for all creatine kinase activity (including CK-BB and CK-MM). Purity for each lot is also reported by SDS-PAGE.

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Catalog Number   C1232
Part Number   90673
Source   Human CKMB produced in E. coli
Form   Clear liquid - Buffer: 5 mM Sodium Succinate, 10mM Sodium Chloride, 1mM EDTA, 5mM β-Mercaptoethanol, 50% Glycerol, pH 7.0 ± 0.1
Purity
Specification
  Purity ≥99% by agarose electrophoresis when stained for creatine kinase activity (report method)
Report purity by SDS-PAGE
Protein / Content Specification   ≥0.5 mg/ml by immunoassay (report method)
Report protein concentration by Bradford Assay
Activity Specification   Report units/ml at 37°C by CK-NAC Assay
Report units/mg CKMB
One unit converts 1 μmole of creatine phosphate to creatine per minute at the specified temperature
Storage / Shipping   Product is shipped using a refrigerated gel pack (not temperature monitored). On arrival, store refrigerated at 2°C to 8°C short term (up to 2 weeks) or frozen between -10°C to -25°C. Avoid repeated freeze-thaw cycles.
Precaution /
Hazard Statement
  This product is not considered a biohazard. Handle using normal laboratory procedures.
Intended Use   For Research Use or Further Manufacture Only. Not for Use in Diagnostic Procedures.
Catalog Number:
C1232
Part Number:
90673
Physical Form:
Clear liquid
Purity:
Partially pure. Purity reported by SDS-PAGE.
Creatine Kinase Purity:
≥99% CK-MB by agarose electrophoresis when stained for creatine kinase activity.