Temperature & pH Dependence of Tris Buffer

pH of 50 mM Tris-HCl Buffer

______4°C____________25°C____________37°C______
7.79 7.20 6.91
7.92 7.30 7.02
8.00 7.40 7.12
8.10 7.50 7.22
8.21 7.60 7.30
8.29 7.70 7.40
8.40 7.80 7.52
8.51 7.90 7.62
8.61 8.00 7.71
8.71 8.10 7.80
8.81 8.20 7.91
8.91 8.30 8.01
9.01 8.40 8.10
9.12 8.50 8.22
9.21 8.60 8.31
9.31 8.70 8.42
9.39 8.80 8.51
9.50 8.90 8.62
9.59 9.00 8.70