Scripps Laboratories

Temperature & pH Dependence of Tris Buffer

pH of 50 mM Tris-HCl Buffer

______4°C____________25°C____________37°C______
7.79 7.20 6.91
7.92 7.30 7.02
8.00 7.40 7.12
8.10 7.50 7.22
8.21 7.60 7.30
8.29 7.70 7.40
8.40 7.80 7.52
8.51 7.90 7.62
8.61 8.00 7.71
8.71 8.10 7.80
8.81 8.20 7.91
8.91 8.30 8.01
9.01 8.40 8.10
9.12 8.50 8.22
9.21 8.60 8.31
9.31 8.70 8.42
9.39 8.80 8.51
9.50 8.90 8.62
9.59 9.00 8.70

Technical Info

Reagent / Buffer
Data